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Holt et al. (2005) used targeted microarrays to track transcript expression in the rat IC at 3, 21 and 90 days after bilateral deafening.
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This makes it possible to track transcripts of any conversation — including rich media such as video, audio, location and images — and compare live conversations with historical ones.
The Cuffcompare (version 0.0.5) tool was applied to track transcripts across the time-points (4, 8 and 14 weeks of nodule age) and comparison of assembled transcripts to reference annotation.
These 5680 tracked transcripts had RPKM ≥1 across all 12 samples (six mRNA-seq, six ribosome footprint).
(C ) Translational efficiency spans >10-fold across all tracked transcripts without any large shifts across time points.
Genes curated from the literature related to ER stress (left) and apoptosis (right) that were found in among the ∼5700 tracked transcripts.
(C ) Translational efficiency (TE) for all tracked transcripts as measured by (RPKM of footprint sample/RPKM of mRNA-seq sample) at each time point.
The coverage track shows transcript accumulation at four of the seven predicted exons in the Glyma1.01 gene model for Glyma20 g18880 but also at several other regions outside the predicted gene model.
We applied the Viterbi algorithm to predict the optimal path of transcript tracks per time point with a 10 nt window resolution.
Thinking about the racial imaginary has also led her to track down transcripts of police killings, "because so many of these videos where somebody says 'Why did you shoot me?" and the person says, 'I don't know.
(A and B ) Paired mRNA and ribosome footprint reads for HSPB1 (Cluster Upreg) and HMGB1 (Cluster Downreg) showing that protein translation generally tracks with transcript abundance.
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