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The software programme NVivo [ 27] was used to aid and track manual analysis and features such as word frequency and other data examination facilities utilised.
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Cell tracking (manual tracking and chemotaxis plugins) and image quantification were performed with ImageJ.
Cell tracking (manual and automatic), and measurements of angles and distances, were performed on videos of projected z-series using Axiovision (Zeiss, North Ryde, NSW, Australia) or ImageJ (National Institutes of Health, USA) software.
For backward tracking algorithm, manual tracking results from representative movies are independently acquired.
Only the cells that migrated within the microscope field were selected and tracked using the "Manual Tracking" plug-in in NIH ImageJ.
EB1 particles in the contrast-enhanced sequence were tracked using the Manual Tracking macro in FIJI (Schindelin et al., 2012).
Cell movements were tracked using the Manual Tracking plug-in in Fiji/ImageJ to monitor the xy position of the centre of the nucleus.
Cells were tracked manually with an ImageJ plugin ("Manual Tracking" Rasband, W.S., ImageJ, U.S. NIH; http://imagej.nih.gov/ij/).
Guidance and control methods were more sophisticated than the original visual tracking and manual commands.
Particle velocity and number findings were validated using image tracking and manual particle counting techniques respectively.
The Nest thermostat also tracks your manual heating adjustments.
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