Exact(1)
A solenoid valve located between the pressure reservoir and the model trachea control the gas released from the tank.
Similar(58)
The modular, bottom up approach used to grow stem cell-based cartilaginous tubes in this report is a promising platform to engineer complex organs (e.g., trachea), with control over tissue size and geometry, and has the potential to be used to generate autologous tissue implants for human clinical applications.
Semiquantitative RT-PCR with equal amounts of cDNA (infected trachea vs. control meaning non-infected material) showed that expression of these representative genes was increased whereas expression of the housekeeping gene rpl 32 was not (Fig. 5).
Viral loads in the lungs and trachea of control monkeys were as high as 50 and 100 TCID50/mL, respectively (Table 2), but rRBD-immunised monkeys could not isolate MERS-CoV from the lungs.
However, at E18.5, the number of tracheal basal cells (P63+) decreased and Clara cells (CC10+) increased in the mutant trachea versus the control trachea.
Using real-time reverse transcription quantitative PCR (RT-qPCR) on RNA extracted from E15.5 tracheas, we observed increased expression of Tbx4 and Tbx5 in the mutant trachea versus the control trachea.
The endotracheal tube was subsequently removed and a cannula was inserted into the distal trachea under visual control.
Semiquantitative RT-PCR was performed with trachea isolated from control animals and those infected with Pseudomonas aeruginosa.
Comparison of trachea in younger, control L3 larvae (40 42 hrs post L2-L3 molthathad had a similar density of cells in the DT revealed that the proximal DB was normally broadened at this earlier stage.
For endotracheal intubation, a flexible cannula (16 ga, 2 inches in length) was placed in the upper trachea under visual control and sealed against outside air with a modified pipette tip around the catheter wedged gently into the laryngeal opening (Kreyling et al. 1993, 2002; Osier and Oberdörster 1997).
As controls, trachea was incubated with cytochalasin B prior to infection with 1 × 10 CFU P. aeruginosa ATCC 27853.
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