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Potentially mixed sequence traces were verified by re-amplification and sequencing.
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The functional activities of this intrabody, i.e. target tracing and capturing, were verified in a human embryonic kidney cell line, 293A, which naturally expresses CD147.
The found TSs were verified by tracing along the reaction pathway to find the related pre-reactive species.
Four properties, with increasing complexity, were verified on these traces.
The topographic features were verified for consistency between trace and retrace images, as well as for their reproducibility in subsequent scan frames.
Single nucleotide indels and SNPs occurring in only one strain were verified by visualizing the original trace data.
The mutations include six nonsense SNPs and 16 insertion/deletion events, and were verified by inspecting the capillary sequencing traces and Illumina reads data for Paratyphi A AKU_12601.
Discrepancies were verified by manual examination of the sequence traces.
(P. Green, personal communication) SNP genotypes were verified by manual evaluation of the individual sequence traces.
The relative timings of motor action and sensory stimuli were verified by simultaneous capture on a multiple trace oscilloscope.
Data were verified during interviews with their oncology physicians and their family history was traced back for ≥3 generations and laterally to 2nd and 3rd degree relatives.
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