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Each of the traces was calculated from an average percent recovery of n = 5 cells at each time point, starting at 4 s post bleach.
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Subsequent velocity, acceleration and power traces are calculated from the smoothed position trace.
For each candidate EPSC, given that multiple samples of a convolution trace from one matched filter are likely to exceed the threshold, and considering that multiple convolution traces can exceed the threshold, the centers of mass of all the suprathreshold segments in all convolution traces are calculated.
The mean current and variance of 250 500 traces were calculated.
Average traces were calculated as in Panel G.
Corrected OCR t) traces were calculated using the time constants obtained above (Table 1, bold) in all further experiments.
Normalized, background-subtracted ΔF/F traces were calculated from these according to the equation, (3) (Δ F F ) i = F i F b a s e l i n e − 1 where F i is cell fluorescence on frame- i and F baseline is the median fluorescence intensity of the cell on that trial.
The area under the curve of the time by VO2 and ventilation (VE) traces were calculated for the different periods of the protocol: during sprints, during the recovery between sprints, during the 5 min recovery period as well as total areas under the curve for the entire protocol.
Pillai's Trace was calculated as this is robust to departures from normality.
The autocorrelation function for each isolated position time trace was calculated and later averaged to produce the ensemble autocorrelation function for the transient state.
To assess the ability of the model to accurately reflect constant respiration, we performed regression analysis of OCR t) calculated by the compartment model correction algorithm to a theoretical respiration model where the variance of the OCR t) trace was calculated for the measurement period.
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