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The silver nanocluster modified electrode is used for square wave voltammetric sensing of traces of nitrate.
This suggests, that also in A. nidulans external traces of nitrate in supposedly "nitrate-free" media are accumulating in NR mutants leading to a "pseudo-constitutive" phenotype.
These traces of nitrate, however, apparently are too low to be detected by our methods directly in the growth media or in the concentrated stock solutions and certificates of chemical analysis of the supplier of media components (e.g. l-arginine and l-proline) do not specify nitrate as contamination.
Results from this study instead suggest that transporter-mediated NO 3 - accumulation in NR deficient mutants, originating from traces of nitrate in the media, is responsible for the constitutive expression of NirA-regulated genes, and the associated phenotype is thus termed "pseudo-constitutive".
Similar(56)
The culprit turned out to be 15 gallons of harmless holy water that happened to contain traces of nitrates.
Traces of nitrates were present in the composts from the onset of composting and concentrations increased significantly as composting proceeded (Figure 7).
They are being held on charges of terrorist murder and conspiracy, with Belgian media reporting that traces of ammonium nitrate, a fertiliser that can be used to make explosives, were recovered from their homes.
Takahashi et al. [33] confirmed that only trace amounts of nitrate and ammonium ions can be determined.
Such external trace amounts of nitrate may occur as contaminants of media components which might accumulate over time inside NR-mutant cells by nitrate transporter activity, and eventually lead to activation of NirA.
Although the genes encoding nitrite dissimilation activities have been shown to be strongly induced only under anaerobic conditions, a basal level of activity could be sufficient to remove the small amount of nitrite produced by nitrate reductase in niiAΔ strains growing on media containing trace amounts of nitrate (or nitrite).
Since farmers applied both inorganic and organic fertilizers for agricultural purposes, it would be helpful for future study to apply the nitrogen isotope techniques to trace the source of nitrate contamination.
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