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In the phospholipid:diacylglycerol acyltransferase specific assay microsomes were used as the enzyme source, because only traces of label were incorporated into TAG when homogenates of the respective strains were used as an enzyme source.
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Similar features were described in another study published earlier [ 2] and in which some control cats were also reported with traces of labelling in some glomerular structures of the kidney.
Whereas substantial amounts of labeled TAG were formed in the presence of the phospholipid mixture used for the acyl-CoA independent assay (see Materials and methods), only traces of labeled TAG were formed when individual phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phophatidylserine) were used as acyl-donor.
Hardly any trace of label was found at depth of the CF and PM treatment.
RNA binding experiments were performed using guide concentrations that ranged between 0.08 and 0.64 µM and 0.32 µM of unlabelled target RNA spiked with a trace of labeled target in 20 µl reaction buffer (30 mM Tris pH 7.5, 150 mM KCl, 5 mM spermidine, 10 mM MgCl2, 0.1 mM dithiothreitol (DTT), and 0.1 mM EDTA pH 7.5) for 5 minutes at 30°C.
"It's like a 'contains traces of nuts' label, so at least you know you're not wasting your money".
Bottom panel: example traces of SemapHluorin labeled DCV fusion events.
Two example traces of kinesin labeled with a GBP-sQD are shown in Figure 2C.
For TLC and autoradiography, Irga6 was incubated with the indicated amounts of unlabeled nucleotide and traces of radioactively labeled nucleotide.
Briefly, trace amounts of labelled RNA (50,000 cpm) were heated at 70°C for 5 min and then slow-cooled to room temperature over 1 hr in buffer containing 100 mM Tris-HCl (pH 7.5) and 100 mM LiCl or KCl of 10 µl.
Volunteers are given tiny traces of a radioactively labelled drug too small to harm them, and typically just a hundredth of the estimated full dose in a drink.
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