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Very recently, these latest results have been challenged, showing that there is no distinct signature conserved across reprogramming experiments (neither at the gene-expression nor at the chromatin mark level for the marks H3K4me3 and H3K27me3) [46], but rather a lab-specific signature [47], or traces of cell memory [48], [49].
Fluorescence traces of cell populations with synchronized oscillations were extracted from CellASIC movies using background corrected mean fluorescence intensity from the entire field of view.
Therefore, attractively, protein interaction modules and their relations with other proteins above can be thought as the dynamical markers (or temporal traces) of cell cycles in phase transitions.
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Traces of cells in the anterior chamber were still observed in OU.
Colonies that left no macroscopic traces of cells after washing were considered noninvasive, whereas colonies that remained on plates after washing were considered invasive.
The time-course trace of cell body migration and neurite tips showed that the neurite tips remained around the tips of the ratchet teeth.
For the non-invasive tracing of cell lineage, lipophilic fluorescence dye DiI (red) and DiD (blue) was randomly microinjected into each of two different blastomeres in each embryo.
Immunostainings by different microglial markers, AIF-1, EMAPII, OX6, P2X4 receptor (P2X4R), indicated that microglia were not fully activated and tracing of cell proliferation by 5-bromo-2´-deoxyuridine revealed that only a small fraction of proliferating cells were microglia (less than 5%).
It will allow temporal and spatial overexpression studies and facilitate bona fide lineage tracing of cell fates.
As for MADM, the required mitotic recombination, although facilitates tracing of cell lineage, makes it less efficient to label differentiated cells.
Tracing of cell cycle kinetics, apoptosis, protein content, and mitochondrial respiration provides additional information on disturbances of cytologic processes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com