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Figure 4 shows a difference plot and sequencing traces for cell line controls, wild-type samples and a selection of patients with KRAS mutations.
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Motility traces for 50 cells on each slide were generated using Videopoint 2.5 software.
(A) Average FRAP traces for metaphase cells expressing GFP-tubulin and either mCherry-clathin light chain a or mCherry-TACC3.
DOI: http://dx.doi.org/10.7554/eLife.01982.006 We first looked for evidence of sustained or sequentially timed neuronal activity in calcium fluorescence traces for individual cells (ΔF/F,'Materials and methods: Data analysis'), on trials following the CR learning peak.
From each wound we quantified single-cell time traces for over 3000 cells.
Panel A shows the ΔF/F, spontaneous activity traces for all the cells in a dataset, sorted according to the correlation clusters they belong to.
Here too, mean spike and eyelid traces for cells split on whether they showed this early focal area or not were in line with this distinction.
In the system studied in this paper the modelling and experiment design was done on average cell traces for R. sphaeroides responses.
Representative whole cell current traces for (F ) are presented in Figure 5 figure supplement 1.
I'm gonna ask Padraig to put these into the NetPyNE code generator and will let you know what happens! we've implemented the changes you've suggested and tried to save spike only from 100 cells and traces for ~40 but I still get the same error!
For the non-invasive tracing of cell lineage, lipophilic fluorescence dye DiI (red) and DiD (blue) was randomly microinjected into each of two different blastomeres in each embryo.
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