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By subtracting currents traces evoked by this protocol, the A-type K+ can be isolated [37], [38].
Membrane currents were compensated offline for stimulus artifacts using Igor Pro 6 software according to the following procedure: Ten traces evoked by voltage steps from −80 to −70 mV were averaged and fitted monoexponentially.
Membrane capacitance and input resistance were obtained from current traces evoked by hyperpolarizing steps (10 mV).
Membrane capacitance and input resistance were obtained from current traces evoked by a hyperpolarizing step of 10 mV.
(C, D ) Whole cell current responses (top traces) evoked by graded deflections of the stereocilia bundles (bottom traces) in IHCs (C ) and OHCs (D ) in Myo15 +/ΔN (left) and Myo15 ΔN/ΔN (right) hair cells.
(A ) EPSC (−60 mV holding potential) traces evoked by low-frequency stimulation and by high-frequency spike bursts under control and 10 min after 10 µM baclofen application in acute hippocampal slices.
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Leakage and capacitive currents were subtracted digitally off-line by scaling the trace evoked by voltage steps from −20 to −30 mV.
Whole-cell current traces were evoked every 5 s by a 100-ms depolarization to the voltage corresponding to the maximal activation of the NaV-subtype in control conditions, starting from a holding potential of −90 mV.
Figure 1 Illustrative traces of visual evoked potentials obtained by checkerboard (left column), windmill-dartboard (middle column) and partial-windmill (right column) stimulation in a healthy volunteer (upper row) and a migraine without aura patient between attacks (lower row).
In the training stage, the user identifies raw experimental data traces that contain evoked EPSCs that are sufficiently strong with respect to background noise; and the user is prompted to manually mark evoked EPSCs.
(E ) Representative traces of EPSCs evoked by paired-pulse stimulation.
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