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Cells co-labeled for MAP-2 and GluR1 were considered neurons, and analysis lines drawn around the soma and one randomly selected neurite per field to determine mean intensity of staining within the traced region.
The Tom-Tract and MT1-pHLuorin signal at each time point was background corrected at each invadopodium with an identical traced region placed in a similar area of the cell but lacking an invadopodium.
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The areas within the traced regions were subsequently summed to derive volumes of the KNOB for each hemisphere.
Invadopodia were traced using the actin immunostaining and average intensity of the actin and IQGAP signals within the traced regions was obtained using ImageJ.
The included studies of these reviews used region of interest analysis of brain structures by manually traced regions of interest in predefined brain regions.
Manually traced regions of interest (ROIs) of each major arterial vascular territory (figure 2B D) were identified by two neuroradiologists (DJM and DMM).
In this rostro-caudal block, the traced regions included the hippocampus proper, the fimbria, the dentate gyrus, the presubiculum, and the subiculum.
In the hippocampus and frontal cortex, traced regions of interest included the dentate fascia, the dentate fascia with the hippocampus proper (CA4-subiculum), and the cross-sectional midfrontal gyrus (spanning crest to sulcal depth).
First it segments the picture by tracing regions that are differentiated by color.
In this way, the watershed lines trace regions of high gradients and segment the original image it into connected regions of small gradients.
Measurements of the volume of the meal and of the gas in the stomach were carried out manually by tracing regions of interest on each slice using the Analyze6 software (Biomedical Imaging Resource, Mayo Foundation, Rochester, MN, USA) and summing across the slices to determine the total gastric volume.
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CEO of Professional Science Editing for Scientists @ prosciediting.com