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The process of dominance is not well understood largely because it is not possible to trace the cellular changes that occur within a follicle in real time in order to relate the events preceding development to future outcomes, such as predicting whether an individual follicle will become dominant or subordinate.
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FITC was conjugated on MWCNTs-polyethylene glycol (PEG) and MWCNTs-PEG-TAT to provide fluorescence signal for tracing the cellular uptake of the nanocarrier.
When the researchers further traced the cellular effects of α-KG, they discovered that it indirectly inhibits a protein called TOR that gauges nutrient supplies and, scientists suspect, helps determine how fast we grow old.
Cellular levels of reactive oxygen species (ROS) were measured by H2DCFDA (2′,7′-dichlorodihydrofluoresceinorescein diacetate) staining and autophagy was assessed by tracing the cellular levels of autophagy markers using western blot assays.
Thus, we believe that long-term adaptation leaves traces at the cellular level lasting from several minutes to a few hours.
Here, the patch pipette was filled with the cell-impermeable calcium indicator OGB1 (120 µM) and a morphometric dye, Alexa 594 (20 µM) to allow simultaneous two-photon imaging and anatomical tracing of the cellular processes.
For instance, by sorting cells having rather homogeneous intracellular states from a population of pluripotent cells and later following the development of single cells, one can trace the trajectory of cellular states in the multidimensional phase-space, as depicted in the Figure.
Conventional methods for the detection of cell death cannot trace the 'history' of the cellular demise with respect to mitosis.
Degradation area per cell was determined using MetaMorph software (Molecular Devices, Sunnyvale, CA) by tracing a region around the cellular actin footprint and measuring the thresholded area of dark spots in the FITC-FN in that region.
Although studies in murine models of AC employing the strict lineage tracing methods required to determine the cellular source of adipocytes have yet to be performed, studies in skeletal muscle (SM) further support our hypothesis in implicating Sca1+ PDGFRα+ tissue-resident mesenchymal progenitors.
But most links are so obscure that even when the chemical basis has been traced all the way down to the cellular level, the actual benefit is far from clear.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com