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Glycerol feeding was then initiated to increase the cell biomass under limited conditions: 500 mL of 50% glycerol containing 6 mL of PTM trace salts was fed at 60 mL/h.
In this way, the membrane permeances with respect to single ions (Na+, Cl−, NH+4, NO−3) were obtained for various concentrations of both dominant (0.05 0.3 M NaCl) and trace salts (0.001 0.003 M NH4Cl/NaNO3).
The shake flask culture was used to inoculate an 30-L fermenter (Bioengineering, AG) containing 20 L of fermentation basal salts medium FM22 supplemented with PTM1 trace salts (1.1 mL of stock solution/L) and biotin (0.4 mL of the stock solution/L).
The composition of the trace salts solution was the same as described previously [ 32].
Trace salts and vitamins were added, and the medium was filter sterilized.
After sterilization, 4.35 mL/L PTM1 trace salts and 2 mL Antifoam 204 were added to the medium.
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The fluorescence of DADNC is highly sensitive to trace salt ions and ionic organic contaminants.
The surface activity and high sensitivity to trace salt ions and organic contaminants were measured.
The mineral salt medium (MM) used for enrichment and growth was modified from the one used by Fortnagel and coworkers (1990), and comprised (per liter): 3.5 g of Na2HPO4.2H2O, 1.0 g of KH2PO4, 0.5 g of (NH4 2SO4; 0.1 g of MgCl2.6H2O, 50 mg of Ca(NO3 2.4H2O, 1 mL of vitamin B12, and 1 mL of trace salt solution.
A novel amphiphilic fluorescent sensor bearing high surface activity, 4- 3-Dimethyl-benzyl propylamine ammonium)-N-dodecyl-1,8-naphthalimide chloride (DADNC), has been designed and used to rapidly detect trace salt in the pure water as an "on-off" fluorescent sensor.
Batch cultivations were performed in basal salt medium, containing (per L): 26.7 mL H3PO4 (85%), 0.93 g CaSO4, 18.2 g K2SO4, 14.9 g MgSO4·7H2O, 4.13 g KOH, 40.0 g glycerol, 0.5 mL antifoam, and 4.35 mL trace salt stock solution.
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