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Implementation of the TRACE protocol to the CPR process was feasible, required minimal interruption of cardiac compressions and resulted in a high recognition rate for the cause of OHCA.
Extraction was based on the protocol of the investigator kit (Qiagen) for extraction of blood and tissue samples, with modifications as follows: tissue samples (about 0.5 cm × 1 mm) were digested with 15 μL proteinase K in 290 μL lysis buffer (included in the kit) up to 4 hours and treated with the "trace" protocol.
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This dual tracing protocol was further implemented with the visualization of different subpopulations of striatal interneurons.
This inexpensive method can be developed as a source tracing protocol when unexpected plague outbreaks or bioterrorism attacks happen.
A manual tracing protocol for identifying and measuring the hippocampus on T1-weighted images was adapted from Watson et al [21] and has been published before [22].
The New Zealand Ministry of Health introduced a contact tracing protocol shortly after this flight arrived in NZ.
The urban dwellers were more difficult to trace and the fourth phase of the tracing protocol was frequently needed for these participants.
An internationally standardized contact tracing protocol, communicated with the International Civil Aviation Organization (ICAO) and International Air Transport Association IATAA), would facilitate the timeliness, and therefore effectiveness of contact tracing.
carried out a manual tracing protocol based on the published standardized guidelines (Nacewicz et al. 2006; Pruessner et al. 2000; Szeszko et al. 2004).
The DSC value of the right hemispheres of four subjects traced twice ranged from 0.86 to 0.91 indicating very good agreement and confirmed the reproducibility of the tracing protocol.
16 Given its structural complexity and functional specificity, the obtained hypothalamic volume was divided into 2 equal volumes in the anterior-posterior axis (see Supplementary Information for detailed description of the tracing protocol and Supplementary Fig 1).
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