(B ) Trace of time-dependent changes in the ratio of reduced to oxidized roGFP2 detected optically as the ratio between the emission signal (at 535 nm) upon excitation at 405 nm vs 488 nm (ex 405/488) following introduction of the fully reduced probe into the indicated solutions of oxidized glutathione (GSSG) or PDI and GSSG.
(E ) Trace of time-dependent changes in the ratio of oxidized and reduced roGFP2 and Grx1-roGFP2 probes expressed in the ER of HeLa cells following a brief (1 min) reductive pulse with dithiothreitol (DTT, 2 mM) followed by a washout.
(G ) Trace of time-dependent changes in the ratio of oxidized to reduced probes expressed in the ER of HeLa cells alongside active or inactive ChaC1 (tagged at its C-terminus with mCherry to allow visualization of cells co-expressing the redox probes and the glutathione-depleting enzyme) following a brief reductive pulse with dithiothreitol and a washout.
(F ) Trace of time-dependent changes in the ratio of oxidized and reduced roGFP2 expressed in the ER of mouse embryonic fibroblasts with genetic lesions compromising disulfide bond formation (Ero1am/m; Ero1bm/m; Prdx4m/y) following a brief reductive pulse with dithiothreitol followed by a washout.
Images are now mere traces of time in motion.
Rembrandt's self-portraits change, as he closely monitors the traces of time.
"In museums," Mr. Kalka said, "ordinary objects become more interesting exactly because of traces of time, such as mold, that give them a meaningful context.
The two men realized the instant images were important to their "Traces of Time" project and started to photograph them before giving them to their hosts.
Figure 4 Decay traces of time-resolved PL.
The change in FRET efficiency varied widely between cells (see Fig. S3 for the traces of time courses from individual cells).
There is hardly a trace of Times Square's seedy past, except, perhaps, for Antonio Tangarife, a 51-year-old musician from Colombia who stands, somber-faced, on Seventh Avenue near 45th Street, handing out cards advertising an Eighth Avenue strip club.
