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(G ) Trace of time-dependent changes in the ratio of oxidized to reduced probes expressed in the ER of HeLa cells alongside active or inactive ChaC1 (tagged at its C-terminus with mCherry to allow visualization of cells co-expressing the redox probes and the glutathione-depleting enzyme) following a brief reductive pulse with dithiothreitol and a washout.
(B ) Trace of time-dependent changes in the ratio of reduced to oxidized roGFP2 detected optically as the ratio between the emission signal (at 535 nm) upon excitation at 405 nm vs 488 nm (ex 405/488) following introduction of the fully reduced probe into the indicated solutions of oxidized glutathione (GSSG) or PDI and GSSG.
(E ) Trace of time-dependent changes in the ratio of oxidized and reduced roGFP2 and Grx1-roGFP2 probes expressed in the ER of HeLa cells following a brief (1 min) reductive pulse with dithiothreitol (DTT, 2 mM) followed by a washout.
(F ) Trace of time-dependent changes in the ratio of oxidized and reduced roGFP2 expressed in the ER of mouse embryonic fibroblasts with genetic lesions compromising disulfide bond formation (Ero1am/m; Ero1bm/m; Prdx4m/y) following a brief reductive pulse with dithiothreitol followed by a washout.
timeDepEffects: List of time dependent variables.
The physiology of the essential trace element zinc has been studied extensively in human subjects using kinetic analysis of time-dependent measurements of administered zinc tracers.
The choice of the fitting range was set by the software program (Time Trace Analysis by PicoQuant GmbH) by considering the decay part of the time dependent data according to optimal parameters.
LnCaP-LN3 prostate cancer cells were grown in [U-C]-glucose to trace the time-dependent fate of individual C atoms in different metabolic subunits of UDP-GlcNAc.
Images are now mere traces of time in motion.
Using this "timer", we have successfully traced the time-dependent activity of myo-3 promoter which drives expression in body wall muscle and vulval muscle.
In one account, the shift from hippocampal to cortical representation reflects a transfer of the memory trace through a time-dependent process known as systems consolidation (Squire, 1992; Alvarez and Squire, 1994).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com