Sentence examples for trace of serum from inspiring English sources

Cells were detached with trypsin, washed three times with PBS to remove any trace of serum, and resuspended in a serum-free medium containing 10 μg/mL of bovine transferrin (SFM-Trf).

To evaluate gE2-14 expression by the BoHV-4CMV-IgKE2-14ΔTK BoHV-4CMV-IgKE2-14ΔTK BoHV-4CMV-IgKE2-14ΔTK BoHV-4CMV-IgKE2-14ΔTKD, PEB, MDBK and BoMac cells were acutely infectionwith 5 m.o.i. of BoHV-4CMV-IgKE2-14ΔTK; at 6 h poseveralction, the medium was removed, cells were gently washed with serum-free medium to eliminate any trace of serum protein and incubovineor 72 h with new serum-free medium.

The cells were rinsed with medium to remove traces of serum, which may inhibit action of trypsin.

To split, the cultures are briefly rinsed with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor and thereafter treated with that solution.

Before the final 16 hour serum-free treatment incubation, the cells were washed five times with serum-free media to remove any traces of serum.

Briefly, ES cells were washed to remove all traces of serum and then plated onto gelatin-coated tissue culture plastic in N2B27 serum-free medium.

To prepare CM, semiconfluent monolayers growing in 35 mm plastic Petri dishes were washed extensively with PBS to eliminate traces of serum.

The cell layer was rinsed with 0.25% (w/v) trypsin 0.53 mM EDTA solution to remove all traces of serum which may contain trypsin inhibitor.

At the beginning of each experiment, chondrocytes or OA synovial fibroblasts were washed three times with Hanks balanced salt solution (Cellgro) to remove traces of serum, and placed in DMEM containing 0.2% lactalbumin hydrolysate (Invitrogen, Carlsbad, CA, USA).

The presence of these apparent traces of serum p53 protein concentrations could not be related either to the p53 protein expression status of the primary lung tumours or to the tumour stage, grade or histological type.

After 4 to 5 days of culturing, the confluent cell monolayer was briefly rinsed with 1× PBS pH 7.4 (Gibco, Invitrogen Corporation, Grand Island, NY, USA) to remove all traces of serum.