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Five video clips have been selected from the open video trace library [16] for testing.
Here, we consider H.264/AVC video sequences of Tokyo Olympics from the video trace library [16].
Figure 2 The video quality for the MSS under various numbers of video layers (the video, foreman, is downloaded from the video trace library [27]).
As an example, we take a video sequence Tokyo Olympics coded with single layer H.264/AVC from the video trace library of Arizona State University [16].
As in the previous examples, we used publicly available videos in YUV 4 4 4 color CIF (352 × 288, progressive) format with around 300 frames each, downloaded from the Video Trace Library [42] and from the Consumer Digital Video Library [43].
At the e-value cutoff of 1e-6, about 13% of the L. stagnalis cDNAs have a hit in the A. californica trace library (Table 3).
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All twelve trace libraries were sequenced in a single bulk run of our GS FLX 454 DNA pyrosequencer.
While this eliminates some of the problems related to SYBR Green, it was not applied to trace libraries and suffers from the same drawbacks as all real-time assays.
We therefore used the Celera genomic dataset and EST trace libraries to assemble a rat genomic annotated region as described in Supplementary Methods.
The mean CV for UT-digital PCR assay was found to be 11.8 ± 1.5%, consistent with the results obtained by UT-digital PCR for the trace libraries and significantly lower than the CV measured for UT-quantitative PCR, 21.2 ± 2.6% (p < 0.05, t-test, Figure 3B).
The sensitivity of the UT-digital PCR assay is demonstrated in Figure 2, where two trace libraries, undetectable at stock concentration by UV spectrophotometry or capillary gel electrophoresis, were accurately quantified at dilutions of 1 100 and 1 1000 by UT-digital PCR.
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