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At 5 DPA, no LWM-GS genes could be detected, and both LMW-GS and HMW-GS showed trace expression levels.
In our study, five protein spots were identified as class II chitinases; all exhibited trace expression levels during early grain development, but accumulated during grain filling in both cultivars.
In general, storage proteins including globulins (spots 70, 71, 80 and 82), gliadins (spots 74, 75, 76 and 77) and glutenins (spot 78), triticins (spots 67 and 68) and avenin-like protein (spot 73) accumulated significantly at the later developmental stages, but only had trace expression levels during the earlier developmental stages in both cultivars.
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We further investigated whether established disease characteristics can be traced backward to BCAR3 expression levels in primary breast tumors.
Despite high MrTPS3 expression levels, only trace amounts of germacrene A were detected in floral tissues as thermally rearranged β-elemene (Table 1, Figure 4).
The statistical issues become even more pronounced when transitioning from static microarray data to temporal microarray experiments where the gene expression levels are traced over a period of time.
Clustering of traces according to H2B-Venus expression levels identified two distinct classes of cells (Fig. 3F, Fig. S6).
Starting from the 5 9 somites stage the expression levels of zfper1b started to increase significantly (blue trace and one-way ANOVA P < 0.001) following the temperature shift when compared to control embryos maintained for the entire experiment at 29°C (black trace).
In addition, microRNA expression levels in the lymphoblastic cells were analyzed to trace variants that might alter miRNA expression and explain partly an inherited genetic predisposion to PC. Seventeen genes were selected for resequencing based on the NMD array, but no truncating mutations were found.
MrTPS2 was not expressed in ray florets and showed only trace expression in disk florets.
These data show that the signal sequence has a significant effect on expression and/or subsequent incorporation of the 5-HT3Br1 subunintonto functional receptors, and support the expression hypothesis proposed above (different relative expression levels of homomeric and heteromeric receptors) to explain the different traces in 5-HT3AB and 5-HT3ABr1 receptors.
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