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64 unique TRFs were observed, with between 12 and 24 recorded in an individual trace (data not shown).
The convergence of the Markov chain was quick based on the log-likelihood trace (data not shown) We also calculated the length distribution and the expected breakpoint usages for this modified distribution.
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Using the LRI-HRI program, no significant motif-repeats were found in these recordings; that is, we failed to find a significant difference in the number of motif-repeats between original and randomized traces (data not shown), replicating the results of Mokeichev et al., (2007).
The sex distribution and age group distribution was comparable in those that could be traced and those that could not be traced (data not shown).
We used nebulization parameters that produced cDNA fragments of, on average, 700 bp as assessed by bioanalyzer traces (data not shown).
Full sequencing of the two introns was not attempted due to their length: 7,729 bp and 11,312 bp for introns 1 and 2, respectively, in human, with even longer introns present in savanna elephant based on genomic traces (data not shown).
iv) Finally, any variation observed for a given marker among the repeated experiments was only slightly affected by the quality of sequence traces (data not shown), an observation indicating the "robustness" of the method as specifically evaluated in the next section.
Sequence differences of 14 of the mutations presented in Table 1A were confirmed by examination of the trace data (data not shown).
In addition, isobutyraldehyde was detected only in trace amounts (data not shown), indicating that the activity of AdhA toward isobutyraldehyde is relatively high.
That at least some of the cDNAs fall into the latter category, is supported by the identification of sequences exhibiting 100% identity with 4 of these cDNA sequences, in the genome project's WGS trace archive (data not shown).
Tracing success declined from 66% (418/631) to 36% (20/55) from first to second tracing attempt (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com