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A parasitic passivation coating impedance was successfully minimized by designing electrodes with either a thicker passivation layer or a smaller lead trace area.

It was observed that the passivated lead trace area to passivation coating thickness ratio has a critical value of 5.5, under which the impedance contribution of the coating is minimized.

However, as silicon appears irreplaceable for smart analytical micro-systems, the paper discusses the key aspects for an optimal design of the chips in terms of metal trace area, pad size, thickness of the insulating layers and bulk resistivity.

Here we need to mention that physical reasons behind the residual traces are not yet clear; however, the visible trace area can be considered proportional to the real contact area.

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Figure 6 Comparison of contact areas calculated from experimentally measured friction force and trace areas.

In the next step, we compared contact areas calculated from experimentally measured friction force for one set of NDs using Equation 7 (Figure 6, Manip) and trace areas for another set of NDs (Figure 6, Traces).

Experimentally observed trace areas remained after ND displacement; contact areas calculated for the same NDs according to the FDM (Equation 3) and DMT (Equation 6) approaches using radii of ND end bulbs, measured in SEM, are shown in Figure 6.

Volume is estimated using ImageJ to trace areas of interest in CT slices, then multiply by slice thickness [38].

The tribological and anti-corrosion results indicated that composite coatings showed a lower friction coefficient, wear traces area and higher anti-corrosion in comparison with neat epoxy, owing to the balance of reinforcement, lubrication and barrier properties of nanofillers and cracks generated by them, and optimal additive concentration of FC60 and FG both were 0.5 wt.%.

Volumes were calculated by multiplying the traced area on each slice by slice thickness, and then summing volumes across slices.

For quantitative analysis of Fluorojade C labeling, the peri-lesion area was traced and 400 μm grids were randomly placed throughout the traced area.

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