Exact(1)
The current paper provides answers to a group of pivotal questions related to the structure/function complex of the odontocete TPC: 1) what are the probable sites and mechanisms for acoustic stimulation of the TPC? 2) Do these results suggest whether or not the ossicular chain is functional?
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Accordingly, gene/functional silencing of either RET/PTC1 or MET abrogated early branching morphogenesis in TPC-1 cells.
Further, the resident human peripheral nucleated cells incubated in the presence of iC3b-ABP-F complexes inhibited the proliferation of human tumor cell line TPC-1 in vitro.
Both in transformed thyrocytes and in the human PTC cell line TPC-1, Ret/ptc1-Y451-dependent signaling and Met cooperated to promote a proinvasive phenotype.
FRO, TPC-1 and WRO expressed both filaments as well as PT (data not shown).
This result indicates a correlation between TPC-1 motile behavior and ADAM17/TACE activity.
TPC-1 cells were seeded at 3×104 cells/cm2 and cultured for 4 d.
We also examined TPC-1 cell motility using the scratch assay in presence of the ADAM17/TACE inhibitor CGS.
We further determined whether epidermal growth factor receptor (EGFR) activation influenced sALCAM release via ADAM17/TACE in TPC-1 cells.
However, in the case of TPC-1 cells the anti-ALCAM antibody I/F8 failed to augment cell motility.
Western blot analysis revealed the expression pattern of ALCAM in total cell lysate and in conditioned medium of the TPC-1 cell line (Fig. 1A).
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