Similarly, three sentences later, the statement, "as the tetanus toxin experiments suggested…", also contained multiple concepts.
Since the tetanus toxin experiments suggested that rescue of the ocr-2; npr-1 phenotype by a pASH::ocr-2 transgene did not require synaptic transmission, we speculate that the Ca2+ effects of disrupting ocr-2 are local to the gap junctions and not visible at the RMG cell body.
The spectra obtained by toxin titration experiment (Figure 6A) clearly demonstrate that the protein microenvironment at pH 7.0 was less favourable to accommodate toxin as a chiral conformer.
We repeated the toxin confrontation experiment with Sterigmatocystin in generation 33 and additionally tested sensitivity to Aflatoxin B1 and Ochratoxin A. These are two cytotoxic and carcinogenic mycotoxins that are not synthesized by A. nidulans, but Sterigmatocystin is the penultimate precursor of Aflatoxin B1 in A. flavus and thus of similar chemical structure [ 29].
Discussion: Taken together, the results of the sham and toxin challenge experiments demonstrate that the dosimetry function of the ABES improves the precision and accuracy of inhaled dose delivery and calculation as compared to predictive WBP conducted before the exposure.
We thank Ramin Raouf for help with toxin binding experiments.
For tetanus toxin (TeTx) experiments, stock solution was prepared at 1,000× of final concentration (75 nM, 250 nM) and was added to internal solution just prior to recordings.
In the lethal toxin (LT) experiments, mortality of mice after a lethal dose of LT was performed based on previously described methods [37].
So in summary, our Cholera toxin B experiments indicate a PKCα mediated effect on nephrin endocytosis, however for a clear differentiation of raft- or clathrin-mediated endocytosis additional experiments would be required since Cholera toxin B cannot clearly differentiate both endocytosis types.
In late January 2000, the Centers for Disease Control and Prevention sponsored a workshop to discuss standardizing the laboratory materials and methods used for in vivo fish bioassays and toxin induction experiments.
