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Descriptive variables of patient characteristics and toxicity were calculated directly from the database.
Incidence rates with 95% confidence intervals for toxicity were calculated and compared by initiating regimen type (stavudine-based versus other).
Estimates of the freedom from Grade 2+ (FFGI) GI toxicity and FFG2 GU toxicity were calculated by the Kaplan Meier method.
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Following the 60 minute stretch period, wells were imaged in three locations (20× objective) using a fluorescent microscope (Nikon TE-300, Melville NY), the number of dead and live adherent cells were determined, and toxicity was calculated as the ratio of dead cells divided by the total number of cells (dead plus live cells).
Percent toxicity was calculated on the basis of the absorbance.
Acute toxicity was calculated as per OECD guidelines 420 (fixed dose method) [ 20, 21].
Time to the development of toxicity was calculated using the inverted methods of Kaplan and Meier (1958).
The percentage of toxicity was calculated relative to the control, 2.5×10 cells in medium with no peptide added.
However, we suspect that these cell populations may be pure progenitor cells, since flow-associated toxicity is calculated to be as high as 75% (Additional file 4D).
For this purpose, groups of patients with different AUC values were formed, and for each of the groups the relative frequency of patients developing toxicity was calculated.
The mean duration of toxicity was calculated without restriction, whereas the mean time spent in REL and TWiST was restricted to the clinical follow-up period.
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