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Risk of chemotherapy toxicity was calculated (low, medium, or high risk) on the basis of the prediction model before the start of chemotherapy.
Following the 60 minute stretch period, wells were imaged in three locations (20× objective) using a fluorescent microscope (Nikon TE-300, Melville NY), the number of dead and live adherent cells were determined, and toxicity was calculated as the ratio of dead cells divided by the total number of cells (dead plus live cells).
Percent toxicity was calculated on the basis of the absorbance.
Acute toxicity was calculated as per OECD guidelines 420 (fixed dose method) [ 20, 21].
Time to the development of toxicity was calculated using the inverted methods of Kaplan and Meier (1958).
The percentage of toxicity was calculated relative to the control, 2.5×10 cells in medium with no peptide added.
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The biologically effective doses (BED) using α/β = 2 Gy for late spinal cord toxicity were calculated and normalized to a 2-Gy equivalent dose (nBED = Gy(2/2)).The initial conventional radiotherapy nBED ranged from ~30 to 50 Gy(2/2) (median ~40 Gy(2/2)).
Descriptive variables of patient characteristics and toxicity were calculated directly from the database.
Estimates of the freedom from Grade 2+ (FFGI) GI toxicity and FFG2 GU toxicity were calculated by the Kaplan Meier method.
Incidence rates with 95% confidence intervals for toxicity were calculated and compared by initiating regimen type (stavudine-based versus other).
However, we suspect that these cell populations may be pure progenitor cells, since flow-associated toxicity is calculated to be as high as 75% (Additional file 4D).
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