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Toxic samples are often not made during the hospitalization.
This effect was more evident at high EC50 values, when slightly or not toxic samples were assessed.
After a statistical analysis, concentrations of Sn, Zn, Hg, Cu and Cr (among all physico-chemical analyzed parameters), in order of importance, were the most important factors that divided the two groups of samples (more and less toxic samples).
The hook of "Toxic" samples a portion of "Tere Mere Beech Mein", from the soundtrack of the 1981 Hindi film Ek Duuje Ke Liye.
Phylogenetic trees generated using both markers showed the same result that all of our toxic samples (n = 5) formed a closely related clade that was distinct from the additional non- weakly-toxic Palythoa spp. (n = 3) and the non- weakly-toxic Zoanthus spp. (n = 8) (Fig. 2A,B).
Specifically, the most toxic samples were from the sites during seasons with the largest contributions of diesel and gasoline emissions, whereas wood burning was only weakly correlated with toxicity end points.
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Addition of the α-sheet designs to a pre-aggregated (6 hr), toxic sample of Aβ showed essentially complete inhibition of 97% for α1 and 96% for α3, compared with the extent of remaining aggregation observed in the absence of inhibitor.
Validation of the primers on Jatropha curcas indicated specificity of 3 RAPD primers to toxic genotypes, 5 RAPD and 2 ISSR primers to non-toxic samples and 4 RAPD primers to Mexican samples (Basha and Sujatha 2007).
The mean number of viable spores in toxic leaf litter was about 400 times that in non-toxic samples.
Figure 3 shows the number of viable spores of Bti per gram of dry matter, counted in either non-toxic (6 samples) or toxic (3 samples) leaf litter samples.
Such outcomes support the idea that EDA investigations of toxic environmental samples would benefit of the application of endpoint- and mechanism-specific methods with zebrafish.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com