Sentence examples for touchdown protocol from inspiring English sources

In this touchdown protocol, the annealing temperature was uniformly decreased from 65 °C to 45 °C at the rate of 1 °C per cycle.

Platinum-Taq was used to amplify segments with a 58°C touchdown protocol in presence of 0.4 µM primer, 100 µM dNTPs, 2.5 mM Mg and 1 mM Betaine.

PCRs for HPV16 E7 and the GAPDH gene were performed by a touchdown protocol with the following cycling conditions: 10 min at 95°C (initial denaturation), 6 cycles of step-down PCR consisting of 45 s at 95°C (denaturation), 60 s at 58°C (annealing) – decrease 1°C each cycle until 53°C; and 120 s at 72°C (extension).

PCR reactions were carried out using LaJeunesse (personal communication) "Touchdown" protocol, in a MyCycler thermocycler (BioRad) under the following conditions: an initial denature period at 92° for 3 min, followed by 35 cycles of 30 sec at 92°C, 40 sec at 48°C and 30 sec at 72°C and a final extension period of 10 min at 72°C.

For ospA and ospC, the first round of amplification was carried out using a touchdown protocol; after an initial denaturation step of 95°C for 5 min, 2 cycles of 95°C for 1 min, 64°C for 1 min, 72°C for 1 min were run, followed by decreasing the annealing temperature by 1°C per 2 cycles until reaching an annealing temperature of 55°C, used for the next 17 cycles.

The PCR was optimized through touchdown protocol.

Thermocycling was often carried out using the "touchdown" protocols with annealing temperatures ranging from 65°C to 45°C, (depending on the primer melting temperature) and a range of cycles from 30 to 45 were performed.

Initially, the standard GP5+/GP6+ assay cycling conditions and four different touchdown protocols were compared.

Four touchdown protocols were tested to assess how a touchdown annealing approach affects the amplification of HPV sequences.

Touchdown protocols (Table 1) with starting annealing temperatures of 45°C, 50°C, or 55°C, decreasing by 0.5°C or 1.0°C decrements per PCR cycle down to 40°C were evaluated.

Optimization of PCR conditions was done by adjusting MgCl2 concentrations (2.5 or 3.0 m m) and/or annealing temperature (Ta) with touchdown protocols (Korbie and Mattick 2008).