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Iron (II) and iron (total) were determined using colorimetric methods with FerroZine [36].
Of the obtained focal mechanisms, 99 were determined using more than 100 P-wave polarity data points, and 664, which is 90%% of the total, were determined using more than 30 P-wave polarity data points.
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The concentration and purity of the isolated total RNA were determined using a NanoDrop ND-1000 SpecThermotometer (Thermo scientific).
The concentrations of total RNA were determined using a spectrophotometer.
Total phenolics were determined using the Folin-Ciocalteu reagent [ 13].
Triglycerides and total cholesterol were determined using standard enzymatic methods (Roche Chemicals, Indianapolis, IN).
The contents of total chlorophyll were determined using a modified method described by Sükran [ 55].
The total flavonoids were determined using a colorimetric method as described previously [ 17].
Total proteins were determined using a Bicinchoninic Acid Protein Assay Kit (Pierce/Thermo Fisher Scientific) and denatured using the standard method.
Total phenols were determined using the Folin-Ciocalteu method, modified as described by Barbolan et al. (2003).
Then, quantification and quality of total RNA were determined using a NanoDrop spectrophotometer (NanoDrop Technologies) and agarose gel electrophoresis, respectively.
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