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The total volume of tested solution in each well was 150 μL.
The total volume of tested solution in each well was also 150 μL.
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The total volume of the test chamber was calculated as 1278.2 cm3.
The total volume of the test loop is about 288 L, and only 60 L of the working fluid is circulated in the test loop.
These 33 laboratories represented 19.3% of the total volume of approximately 200,000 tests analyzed in this study.
Effector and target cells were mixed at an E T ratio of 100 1 (2 × 10 for effector cells and 2 × 10 for target cells, respectively) in a total volume of 200 μl per test.
A minimum volume of 110 μL (100 μL for NS1 cassette and 10 μL for Ab cassette) was required to perform the RDT, so a minimum total volume of approximately 10 mL per sample tested was required (i.e., 110 μL × 88 = 9.68 mL).
This stock solution is then diluted in PBS to give an absorbance of 0.7 at 730 nm prior to addition of 990 μL to 10 μL of Trolox (for the standard curve) and then to 10 μL of test extract (total volume of 1 mL) and measured after one minute at 730 nm on a Cary 50 MPR in Nunc 24 well plates.
About 50 μl of the dissolved pellet was added to each of the conditions tested in a total volume of 10 ml.
Briefly, different leafy vegetables were tested in reaction bottles (total volume of 500 mL).
On day 1, 500 mL algae with a concentration of 450 \(\mu g/L\) were mixed with water and particles to a total volume of 1 L in four different test bottles (except for the control bottle, which received only water).
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