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Aliquots (0.4 mL total volume) of stock lipids (CHOL/DSPC/DODAP/PEG2000-Cer16 or CHOL/DSPC/DODAP/DSPE-PEG2000-Mal/PEG2000-Cer16) were added to a glass tube.
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The total volume of crude virus stock was quantified and inactivated by mixing with 37% formalin at a 1/4000 ratio, as described previously [ 21, 22], except that inactivation was maintained at 4°C for 1 week with stirring.
Upside Volume: The total volume of all advancing stocks over a given time period, e.g. daily.
Finally, Vero cells seeded in 2 flasks of 75 cm were injected with 1.5 mL of the second passage virus stock in a total volume of 25 mL to generate a third-passage viral stock.
The effects of the stock pH and GuHCl were minimized by always adding 7.5 μL of stock into a final total volume of 1200 μL with final [GuHCl] = 40 mM.
Infections were performed by mixing 4 μg/ml polybrene (Sigma) with 30 μl of virus stock in a total volume of 3 ml of medium.
GSH (5 mM, 10 μL) was added to the GUALY's reagent derivatizing solution (∼970 μL) followed by addition of a metal stock solution (1.7 24 μL) for a total volume of 1 mL.
Both the B. xylophilus solution (50% of the total volume) and drug stock solution of (1% of total volume) were aliquoted into 96-well microplates (e.g., 100 µl B. xylophilus and 2 µl 200 mM stock solution of test compounds in a total volume of 200((l).
The final assay mixture (total volume of 1ml) contained 10 μmol/L of arachidonic acid (10 μl from 1mmol/L stock) and 2 units of 12-Lox (porcine leukocyte enzyme) in 100 mmol/L Tris-HCl buffer (pH 7.4).
Still, the total volume of margin loans at last report was $252 billion, or just 1.7percentt of the value of stocks traded on the New York Stock Exchange and Nasdaq, the two major stock markets.
For the β-GT glucosylation assay, 1 μg of purified DNA was incubated with 25 μM UDP-[H]glucose (125 μM stock) and 5 units of β-GT enzyme in a total volume of 25 μL.
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