Sentence examples for total volume added from inspiring English sources

The total volume added was 12.6 mm3, therefore, the cells will be in contact with 28 cfu/(12.6/1.1)/4800 approximately equal to 3654 bacteria per cell.

After a 20-minute incubation, 1.6 ml antibiotic-free media containing 10% FBS was mixed in and the total volume added to cells at 50% confluence on 60 mm plates.

For keeping the same protein concentration in each fraction, these soluble fractions were then added with 20 mM Tris HCl and 50 mM NaCl at their respective pH values to make the total volume added to each fraction to be 8.88 μl.

Free thiol concentrations in the gels were quantitatively detected by Ellman's assay as described above, accounting for larger gel size (swollen volume = 84.8 μL; add 15.2 μL of PBS to gel in well plate for 100 μL total volume; add 18 μL Ellman's reagent in 900 μL Ellman's buffer to each well).

This lack of sensitivity is because the thoracic vascular compliance is only one-seventh that of the systemic vascular compliance and a change in total body volume adds only a small amount of volume to this compartment.

200 μl of serum free DMEM was added to the DNA Lipofectamine mix, and the total volume was added to the cells.

Volume Price Trend (VPT) A running sum of the day's total volume is added if the market closes positive or subtracted if the market closes lower.

For paired structures both sides were measured and the volumes added to give total structure volume.

AlamarBlue (AbD Serotec, MorphoSys, Raleigh, NC, USA) agent (10% of total volume) was added to each well for 4 hours before fluorometric detection.

Chloroform, 20% of total volume, was added to the mixture which was subsequently centrifuged (13,000 rpm) at 4°C for 15 minutes.

The fibroblast number necessary for the desired concentration in the gel (1x10-5x10 per ml) was resuspended in FCS (10% of total volume) and added to the gel solution on ice under cautious stirring.