The criteria to consider the diagnosis of a DVT were: non-compressibility of the vein (partial or total), direct visualization of the thrombus in the vein lumen and the absence of coloured or continuous Doppler flow.
We can clearly understand from Additional file 1: Table S1 that 76% of the total unique visualization requirements were provided by geotechnical engineers and 38% of the requirements were provided by geologists, with overlapping requirements between groups.
Membranes were stained with Ponceau S solution (0.25% (w/v) Ponceau S (Serva), 40% methanol (Merck), 15% acetic acid (Roth) in H2O] for total protein visualization, briefly washed and then incubated for 30 min in WB blocking buffer (5% non-fat milk, 0.1% Tween-20 in PBS).
After separation the proteins were either stained with Coomassie Brilliant Blue staining (for total protein visualization) or transferred to a PVDF membrane (ImmobilonP, Millipore) by the semi-dry transfer method (Trans-Blot SD Semi-Dry Transfer Cell, Bio-Rad) for Western Blot analysis.
Brains of PrPKO mice take up 45% more 59Fe relative to Wt controls as estimated by total counts and visualization on X-ray film exposed to dry sections (Figure 2B, panels 1 4, black arrows).
The reattachment length was obtained by a flow visualization technique, total pressure probe and shear velocity plots.
Without visualization the total number was 2117.
For the case with visualization, the total number of actions was 1116.
The research team identified a total of 42 unique visualization requirements based on the research methodology outlined in the previous section.
To overcome these difficulties, we prepared fluorescently labeled vimentin for visualization by total internal reflection fluorescence microscopy (TIRFM).
