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For instance, although the TATA-boxes among Arabidopsis genes were contextually positioned at the correct upstream location, their occurrence in such context was established only in less than 30% of the total validated genes.
The n in parentheses shows the number of total validated deletions for each experiment.
However, approximately 20% of the total validated targets were shown to be non-significant using qPCR.
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The characterization of the pure roasted coffee beans and of adulterations profiles was taken by total carbohydrates validated method based on high-performance anion-exchange chromatography with pulsed amperometric detection.
In total, we validated the top predicted sites for JUND binding in 6/7 of the predicted JUND targets, and we validated E2F1 binding sites in 4/6 of the JUND bound promoters.
As gene expression may be influenced by promoter polymorphisms, we included analysis of SNPs in the 11 kb upstream region from exon 1 and found 62 (out of the total 101) validated SNPs.
However, this variability may, at least partially, be explained by the inconsistency in methodologies used as none of surveys used a dietary assessment tool validated for total water intake or beverage and drink intake.
We also picked 6 promoter or TTR regions in total to validate such methylation differentiation between cultivated and wild rice using the traditional bisulfite sequencing method for single genes, and the results conform to those from our whole genome BS-Seq analyses (Additional files 9 10 11 12 13,and 14), indicating the reliability of our BS-Seq results.
Total RNA was extracted from 16 different crab-eating monkey tissues using the Trizol reagent (Invitrogen), and total RNAs were validated by RNA electrophoresis in agarose gels containing formaldehyde.
The MBR studies demonstrated the process effectiveness in removing AOC, BDOC and total aldehydes, and validated the model's predictive capability.
Finally, the MMSE total score is validated to identify cognitive impairment; however, the domain scores have not been validated as standalone measures.
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CEO of Professional Science Editing for Scientists @ prosciediting.com