Sentence examples for total transcriptome sequencing from inspiring English sources

The abundances of the corresponding RNAs as well of C4 cycle associated transcripts were quantified by total transcriptome sequencing.

Total transcriptome sequencing was performed using the strand-specific Illumina protocol, which was used to generate more than 192 million short reads.

The present study extends such analyses by combining genome-wide TSS mapping with total transcriptome sequencing and protein abundance analysis to identify leaderless transcripts and to assess the contribution of leaderless genes to bacterial physiology in a starvation model.

The current version of this database provides the total transcriptome sequences (root + leaf) and leaf (in vitro) sequences and root (in vitro) sequences separately reported in this study, as well as EST sequences produced from our previous study [ 30].

For the floral transcriptome sequencing total RNA was extracted from the T. hassleriana floral tissue using guanidium thiocyanate-phenol-chloroform extraction protocol [ 15].

Instead of using the pooled total RNA for transcriptome sequencing as in AA6 and AM20, first strand cDNA samples were synthesized from the total RNA samples of AA3 and AM22 using Clonetech SMART cDNA synthesis kit (Clonetech, USA) and second strand cDNA synthesis was carried out using Clonetech PCR kit (Clonetech, USA).

For transcriptome sequencing, total RNA was extracted from ∼3.3 × 10 cells using Trizol (Invitrogen, Burlington, ON, Canada), followed by standard phenol/chloroform precipitation and subsequent precipitation using lithium chloride.

The total data output of transcriptome sequencing was 200.8 Mb in control (C), 222.9 Mb in aphids at 2 h (A2), 231.6 Mb in aphids at 24 h (A24), 297.8 Mb in whiteflies at 2 h (W2), and 244.3 Mb in whiteflies at 24 h (W24) of infestation (Table  1).

There are no sequenced dinoflagellate genomes available to aid in estimating the number of genes in D. acuminata, however, transcriptional profiling of the dinoflagellate Alexandrium tamarense identified 30,917 unique gene signatures, suggesting that only a fraction of the total transcriptome of D. acuminata was sequenced [ 29].

For each sample, 25 μg of total RNA were utilized for transcriptome sequencing (2012 samples).

For the low diversity dataset, we sequenced each of the four haplotypes to 6X coverage to achieve total coverage similar to recent transcriptome sequencing projects (23X in [ 11]).