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Identical cultivation maintenance procedures were used for all groups, with weekly feeding followed by total test solution renewal, and the counting and segregation of cocoons.
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The absorbance was calculated as follows for each standard and sample solution: a calibration curve was prepared using the calculated absorbance for the catechin solution, and the total procyanidin in each test solution was calculated from the calibration curve.
A total of 1 mL of taste test solution was placed on the center of the tongue dorsum with a syringe.
The permeation of lidocaine out of each test solution was examined in duplicate per animal, while six horses were investigated in total.
The total volume of tested solution in each well was also 150 μL.
The total volume of tested solution in each well was 150 μL.
§ 862.1165 Catecholamines (total) test system.
§ 862.1175 Cholesterol (total) test system.
Average total test duration was 50.5 h.
The test solution was vigorously shaken.
Tests were conducted under total immersion conditions in 300 ml of the aerated and unstirred test solutions.
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