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Here the number of template representatives was kept to be 20% of total templates.
In Figure 9, we show how the percentages of template representatives selected from the total templates affect phone accuracies for MDTS and MLTS with their respectively compatible distances.
Here we discuss how the three methods of (1) MDTS, (2) MLTS, and (3) template compression performed when using the LLR and KL distances and show the results in Figure 8, where the number of template representatives were kept to be 20% of the total templates for the three cases (further details are discussed in Section 5.3.5).
These data indicate that the proportion of templates amplified varied between 26 61% of the total templates available.
In total, 2,867 and 9,594 (12,461 total) templates were sequenced from the MPB and DPG libraries respectively, yielding 12,119 total ESTs that passed the sequencer's quality control threshold (2,776 from MPB and 9,297 from DPG).
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This was confirmed by examining changes in total template levels with time by qPCR.
Whole-genome amplification was performed on 10 ng of the total template DNA using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare) following the manufacturer's instructions.
The scores for templates utilizing methane PCI and EI are given by the following eqs 1 and 2: 1 2where I j and M j are the S/N and m/ z, respectively, of the jth member of n total template members.
The PCR reaction (50 μl) contained 5 ng of total template DNA (equimolar mixture of each parental gene), 0.3 μM of each primer, 0.2 mM of each dNTP, and 5 IU Taq polymerase.
The total RNA templates were quantified by spectrophotometry and subjected to 1.0% formaldehyde denatured agarose gel electrophoresis.
The quantity of total RNA templates was normalized to β-actin expression (Forward primer: 5'-gct cgt cgt tcg aca acg gct c-3', Reverse primer: 5'-caa aca tga tct ggg tca tct tct c-3').
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