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That the follow-up question wasn't about "Inspector Spacetime" is a total TCA fail, but the questions did quickly shift to "Community," naturally.
Radioactive measurement of the total TCA-insoluble radioactivity minus the alkali-stable TCA-insoluble activity represents the measure of alkali-labile radioactivity in the total TCA precipitate.
The alkali-labile fraction of the total TCA precipitate represents RNA.
As TCA also inhibits the downstream assay, it was neutralised with Tris-acetate (pH 7.75) to bring the total TCA percentage to 0.1% following the manufacturer's direction.
Therefore total TCA cycle activity was also elevated (5.2 ± 0.3 vs 3.2 ± 0.5 μmol/g dry wt/min, P < 0.05), which is not accounted for with a 12% increase in function.
The contribution of blood lactate oxidation to total TCA cycle activity ranged from ∼2 to 8% over the range measured, with glucose providing 92 98% of the total substrate for oxidative metabolism.
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Total calcium (tCa), ionized calcium (iCa), and feed intake were recorded.
In bicarbonate-based hemodialysis, dialysate total calcium (tCa) concentration may have effects on mineral metabolism.
At the end of each time period, the medium was saved and total and TCA precipitable radioactivity was determined.
The Kidney Disease Improving Global Outcomes position states that the measurement of ionized calcium (ICa) level is preferred, but in the clinical setting, due to technical difficulties, total calcium (tCa) level is preferred to ICa.
The following colorimetric methods (Koni Analyser 20i, Finland) were used to determine plasma analytes: total calcium (TCa), arsenazo III; P, ammonium molybdate: creatinine (Cr), Jaffe; albumin, bromocresol purple; TALP, p-nitrophenol; magnesium (Mg), xylidyl blue I; cystatin C (Cys C), immunoprecipitation; bilirubin, diazo coupling; and aspartase transaminase (AST), enzymatic.
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