Quantification of this inhibition is shown in Figure 3c as the ratio of phospho-Stat-5 to total Stat-5.
Our results shown in Fig. 6 revealed that obesity, but not aging (12-month), significantly reduced Ob-R protein expression and its post-receptor signaling STAT-3 phosphorylation without affecting the total STAT-3 expression.
Total STAT-1 levels were similar in peripheral blood subsets of osteosarcoma patients and healthy controls.
Phosphorylation was calculated as the OD ratio of the phosphorylated to total STAT-1 (standardized to non-stimulated HPAEC).
Total STAT 1 protein levels were assayed by rabbit polyclonal antiserum against C-terminal domain of STAT 1 raised in author's lab (S1C).
In addition, the activation of PLD by pervanadate triggered phosphorylation of tyrosine 705 residue on STAT-3, and its phosphorylation was dramatically higher in TPC-1 cells (from papillary carcinoma) that have an endogenous RET/PTC1 than in ARO cells (from anaplastic carcinoma) without alteration of total STAT-3 expression.
More importantly, under these conditions, the immunoblotting with the antibody specific to phosphorylated Y705 of STAT3 showed that the extent of pervanadate-induced STAT3 phosphorylation was also much higher in TPC-1 cells, without alteration of total STAT-3 expression (Fig. 3B, first and second panel).
Mouse mAb anti-phospho-tyrosine STAT-3 (Y705), affinity purified rabbit anti-total STAT-3, STAT-5 and STAT-6 were from Santa Cruz (Santa Fe, CA).
"Of course I do, but I've said time and time again, those things, totals, season stats, are meant to be looked at at the end of the season".
Consider the following list of Pokémon according to EV: [1] You can have a maximum of 255 EVs per stat, and 510 EVs total on all stats.
