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The total sphere activity was generally overestimated but remained within 10% of the reference activity defined by the phantom preparation.
The outer surface area available for transport is assumed = 0.031 cm, and the total sphere water volume = 260 nl.
Results are displayed as total sphere volume in microns or, where possible, mammosphere formation was normalized to the total mammosphere size of one of the Ep-CAM/CD49f main cell populations (i.e. Ep-CAMlow/CD49f+, Ep-CAMhigh/CD49f + cells).
Also, sphere formation in semisolid culture indicated that both PDGF-AA and bpV pic) could increase total sphere number formed from 10 cells (Fig. 4H-c), and bpV pic), alone or with PDGF-AA, can largely increase the number of spheres with diameters longer than 50 μm (Fig. 4H-b).
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Total spheres were then counted.
While the number of total spheres was not affected by Frondoside A except at the highest concentration tested (1.0 μM/l), the sphere size was inhibited by 100, 92, 28, and 1 % in a dose-dependent manner (Fig. 5d).
Human numerosity estimation is then predicted by the total area (occupancy) covered by the influence spheres.
Regional blood flow was then calculated using the following formula [ 14, 17]: Regional blood flow (ml / minute / g ) = (total tissue spheres ) / (tissue weight, g ) × (reference spheres / ml / minute ) ) After completion of the experiment, the animals were anesthetized with ketamine (15 mg/kg) and sacrificed by intravenous injection of 60 ml saturated potassium chloride.
In total, 6 (spheres) × 2 (contrasts) × 4 (reconstruction protocols) × 3 (emission counts) × 10 (replicates) × 2 (matrix sizes) were acquired, making a total of 2,880 objects to be delineated.
In the application of the above channel model, we assume that the specular ray of the received field is free of depolarization (i.e. T p = 1) while polarization of the scattered component is completely random (taking γ0 = −π/2 i.e. the total Poincaré-sphere surface in that case T p is uniformly distributed between 0 and 1).
Sorted SP and non-SP (NSP) cells from bulk cultured LA-N-5 showed no difference in sphere-forming ability, while SP and non-SP cells from LA-N-5 culturedltured with doxorubicin showed enrichment of sphere-forming ability for SP cells and total loss of sphere formation in the non-SP (Fig. 5C).
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