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Based on resampling-observed B73 fragments, the expected number of unique fragments sequenced for a given number of total fragments sequenced incorporates the uneven coverage distribution.
The annotation resulted in 3548 protein coding sequence encoded by 85.1% of the total sequence length.
The next class of transcriptome assembly metrics concerns the intrinsic properties of the assembled sequences including length, coverage (number of bases incorporated in contigs from reads divided by length) and N50 length, defined as the shortest sequence length such that half of the total sequence output length is included in sequences that are shorter.
tepidum sequence incorporated.
(a) Comparison of deduplicated sequences and total sequences reveals the frequency of repeatedly read sequences.
Phylum Actinobacteria represent∼8% of the total sequences.
Following de-replication of identical sequences, the number of unique, abundant sequences (>10% of total sequences per individual) recovered for each individual ranged from zero to six.
This yielded 968,362 total sequences.
Sequences incorporating a single nt substitution showed significant directional skewing from the true sequence.
Reverse transcription being mostly achieved on total RNA, this however was of methodological interest since those sequences incorporate a part of the isotope during the labelling.
Nevertheless, the total NUE sequence remained similar.
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