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For the reduce phase, typically a 0.95% of the total reduce slot is optimum to reduce task.
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Sugar fractions (total, reducing and non-reducing) showed a significant decrease as the senescence progressed from D2 to D5.
Most significant factors pH (4 7), temperature (20 40 °C), initial total reducing sugar conc.
After hydrolysis optimization, the maximum concentration of total reducing sugars (TRS) was 21.68 g L−1.
The response was measured through four response variables: total reducing sugars (TRS) consumption, VFA's, hydrogen and methane production.
The results were obtained based on the analysis of total reducing sugars (TRS) with the DNS method.
The total reducing sugar content was measured using 3,5-dinitrosalicylic acid (DNSA).
This supernatant was used for further analysis to estimate total reducing sugar by DNS method.
The total reducing power was expressed as equivalent of ascorbic acid (AAE).
They revealed that plants with high antioxidant potentials also showed higher total phenolic contents and total reducing capacity.
At the stipulated times, contents were centrifuged at 10,000g for 15 min and supernatant analyzed for total reducing sugars.
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