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As observed by Wei et al. (2011), transfer was highly efficient and ∼80% of the total recipient cells were already labeled after 12 hr of co-incubation.
All the plates are incubated at 37°C for 2 days before assessment of transformation frequency, which is expressed as percentages of transformants (erythromycin-resistant colonies) against total recipient cells per millimeter cell suspension.
For comparison, engraftment of EGFP-positive BM-derived cells was additionally analyzed in female recipient conditioned Under these conditions, 3.3 ± 0.2% (n = 4) and 8.4 ± 3.1% (n = 4) of the total recipient BM-derived cell population was found to be EGFP-positive at 2 and 4 weeks post-BMT, respectively.
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Of the total recipients, 70% are people who have undertaken outstanding work in their communities.
The additional 67,000 clients added from July 2007 to January of this year brought total recipients to 1.86 million, about one in 10 New Yorkers.
They form less than 5% of total recipients of non-contributory benefits - that is, state benefits which are not funded by individual or employer contributions.
As opposed to the debut edition that ran from 1967-1971, LACMA's current iteration is comparatively balanced, with two female artists among six total recipients, including a pair of collaborators.
A website designed to allow welfare recipients to track their ATM withdrawal fees was visited by about 1percentt of the state's total recipients in January 2014, according to the study.
Transformation frequency was expressed as percentage of transformants against total viable recipient cells per milliliter cell suspension.
Despite the barriers challenging enrollment of any HIV+ child into care, the rates of care demonstrated in this study suggest that pediatric ART recipients, as a percentage of total ART recipients, could more closely approach Malawi's 2014 goal of 15% with broader implementation of this strategy.
The share of total organ recipients aged 50 and older has jumped from 28% in 1988 to 60% last year.
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