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The bar graph represents the total reads for the genus Methylomonas for each species/cultivar from a randomly selected 10000 reads from each sample.
Although the total reads for the BART miRNAs exceeded 110,000 in the T10 sample, no BHRF1 miRNA read was detected, consistent with the observation that BHRF1 is specifically expressed in EBV-positive B cells [9], [11].
Upper panel shows the total reads for each sample.
We used uniquely mapped reads (551,136,389; 60% of the total reads) for further analysis.
An in-house developed Perl script was used to identify the total reads for specific transcripts.
In addition, we only used a subset of total reads for R500 to reduce computational requirements.
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We also compared ratio of the number of reads mapped to total read for the two reference sequences, and subtracted values of the mapping frequencies (% mapped reads with consensus sequence minus that of H37Rv) were calculated.
We obtained 14,472 total reads from intestine, 7,532 for pharynx, and 5,185 for body muscle.
Within each family, the proportion of the total reads accounted for by the major variants (>300 reads) are shown in Figure 3 as well as the total number of reads for each family and the number of variants.
Two abundantly expressed families in our transcriptome analysis are represented by the 30.5-kDa (4.35% of total reads encoding for S products) and Hyp8.2 Culicine families (1.30% of total reads encoding for S products).
(A ) For each sample, sequence reads assigned by eSNaPD are expressed as a percentage of total reads obtained for that sample.
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CEO of Professional Science Editing for Scientists @ prosciediting.com