Sentence examples for total prp from inspiring English sources
The use of multi-factor statistical experimental design methodology minimized the vaccine material and laboratory resources required for optimization and validation of an HPLC assay for quantitation of deploymerized and total PRP.
This increase in total PrP is a measure of both PrPc and accumulated PrPsc.
Insoluble PG14 PrP was highly enriched in the final pellet (P3), which contained approximately 60% of total PrP (Fig. 1B).
The increase of total PrP in infected C57BL/6 mice is most likely due to a doubling of the PrPC level [30] in addition to the accumulation of PrPSc; in infected Tga20 mice there is little PrPSc (see below) and possibly no increase of PrPC, hence less increase of total PrP.
Based on the results obtained with NBH, less than 5% of PrPC is expected to be found in the pellet; on the other hand, in diseased brains bona fide PrPC was 5 30% of total PrP.
Total PrP and PrPres were best represented in the temporal cortex and cerebellum while they were present in the least amount least amount in the occipital cortex (Fig. 2B and E).
In addition to the 25 kDa band, another 18 kDa PrP-specific band was detected in the preparations of PrP-KDEL and PrP-3AV (Fig. 3A), which occupied about 46% and 39% of total PrPs after digitalizing the signal intensities (Fig. 3B).
Mild proteolysis process followed by PNGase F digestion showed that in addition to the 25 kDa band that presented in all preparation, an 18 kDa PrP-specific band was detected in the preparations of PrP-A117V and PrP-G114V (Fig. 7A), which occupied about 34% and 37% of total PrPs after digitalizing the signal intensities, respectively (Fig. 7B).
Those in which plaques were absent tended to have higher total PrPd scores (arbitrary units), principally manifested by higher amounts of stellate, perivascular and perivacuolar (white matter astrocyte-associated) PrPd types.
Under optimized conditions of proteolysis which degrade PrPC, ∼85% or ∼20% of total homogenate PrP resists digestion with thermolysin or PK, respectively [25] (Figure 2A and B).
These data show that the majority of prion infectivity in RML brain homogenate is sensitive to these proteases and suggest that these prions may be associated with only a minor fraction of total homogenate PrP [25].
