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The numbers of 7 AC samples were prepared as reference sample, and 7 AC samples were also prepared for each corrosive environment, so that the number of total prepared AC samples is 42.
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Total RNA prepared from Lhx2 expressing cells was used as reference and compared to total RNA prepared from three time-points (36, 72 and 96 hours) after dox withdrawal.
Hence, 22 kinds of samples in total were prepared.
The total number prepared synthetic standards for calibration or validation sets were 25.
After RT-PCR using total RNA prepared from testis, we examined Ace3 mRNA alignment by sequencing.
For the microarray experiment, we used total RNA prepared from shoots, roots, panicles, and callus.
RT-PCR for Apobec3 and Dnd1 transcripts was carried out on total RNA prepared from the E13.5 genital ridges.
Primer extension of endogenous genes was performed using 10 to 20 µg total RNA prepared from non-transfected cells.
or total RNA prepared using TRIzol.
Kidneys were harvested and total protein prepared.
Gene expression by qRT-PCR was examined using total RNA prepared from seed and bud samples.
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