Sentence examples for total population of cells from inspiring English sources
The authors investigated an acute myeloid leukemia wherein the subpopulation being 0.01 1% of the total population of cells could cause leukemia when transplanted to the immunodeficient NOD/SCID (nonobese diabetic-severe combined immunodefficiency) mice.
FCM data were analysed with Kaluza Flow Cytometry Analysis software (Beckman Coulter) to determine the percentage of Nile Red-positive cells and the median fluorescence intensity (MFI), expressing the increase in fluorescence of the total population of cells relative to the autofluorescence.
After three hours, 81% of CPMV-AF488 positive cells were also positive for surface vimentin, compared with 51% of the total population of cells (Figure S2).
If true, then it could have been the case that only a very minor fraction of the total population of cells displayed the sister separation phenotype.
Cultured cells were harvested from 24 well plates by 37°C Tryple (Invitrogen) treatment and then suspended with PBS and we pooled floating cells with harvested cells to give an estimate of the total cell viability across the total population of cells in each well.
Furthermore, the proliferation status of the total population of cells was assessed by MTT colorimetric method.
It must be emphasized that since we are not employing imaging as such, we are measuring the total population of cell-associated species.
Briefly, total populations of cells, including floating and adherent cells, were fixed in 70% ethanol and stained with propidium iodide (50 μg/ml).
The CD3+ cells were gated from the total population of live cells, and the CD8+ cells were gated from the CD3+ cells.
For the quantification of progenitor cells, we analysed the total population of CD34+ cells, which are the general haematopoietic stem cells, and the subpopulation of CD133+/CD34+ cells, the progenitor population that also contains the EPC.
They reduced proliferation in a dose-dependent manner with a higher specificity as compared with the total population of cancer cells and/or healthy stem cells, and they were efficient in inducing cell death.
