Sentence examples for total polymerase from inspiring English sources

Since the misincorporation capability was defined as the ratio of the level of the primer extended beyond the first stop site with the biased NTP pool to the total polymerase activity measured in the reaction with all four NTPs, the misincorporation capability of IAV Pol actually increased at 42°C, compared to that at the lower temperatures.

First, as seen in Figure 2B (1x) for IAV Pol with UTP and Figure 2D for HIV-1 RT with TTP, IAV Pol generated much shorter and fewer RNA products beyond the first stop site than HIV-1 RT even if there was a 2-fold more total polymerase activity of the 1x IAV Pol than HIV-1 RT, which was estimated from the reaction with all four nucleotides present.

Total polymerase chain reaction (PCR) volumes were 50 µl containing: 1 × TaKaRa Premix Ex Taq (TaKaRa Bio), 0.2 µM of each primer (IDT DNA Technologies), and approximately 200 ng of template genomic DNA.

In Figure 4, the total RNA polymerase protein complex and RNA polymerase phosphorylated on key serine residues at position 2 (PS2) and 5 (PS5) are identified by western blotting with densitometry quantification.

In E. coli, only one of the five total DNA polymerases is the high-fidelity and high-processivity replicase (pol III), and the most abundant pol I has high fidelity, but low processivity, and performs short patch synthesis in DNA repair.

Total ribonucleic acid polymerase II activity increased from 19.9 ± 6.0 fmol of tritiated uridine triphosphate incorporated per milligram of protein per hour at 40 days to 123.8 ± 53.0 fmol at 50 days and to 200.9 ± 77.8 fmol at 60 days (P <.01).

Total DNA-extraction, polymerase chain reaction (PCR) and chain termination sequencing followed standard protocols [ 9, 42].

Total DNA extraction, polymerase chain reaction (PCR), and sequencing were performed as previously described (Cordaux et al. 2008).

The methodology for production of cDNA from reverse transcription of total RNA and polymerase chain reaction (PCR) methodologies have been described by Pinheiro et al. [ 2].

The level of S2P (based on a ChIP using the 3E10 antibody) was determined and normalized on the total level of polymerase (based on a ChIP using the 8WG16 antibody).

Partial fragments of cytochrome b (cob) and cytochrome c oxidase subunit 3 (cox3) genes were amplified from T. globosa total DNA by polymerase chain reaction (PCR) using the distinct sets of degenerate primers, 5′-GGNTAYGTNTTACCWTGRGGNCAAATG-3′ and 5′-GGTARRAAATACCAYTCSGGSACSAT-3′, and 5′-CANNTRGTNGAYCCRAGTCCRTGGCC-3′ and 5′-YCAWACWACRTCWACAAARTGCCAATA-3′, respectively.