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Almost half of them (43% of the total pathogens) were detected at particle sizes < 3.3 μm.
Total pathogens (sum of all 7 species/genera) constituted 2.1% (IQR 1.36-3.871.36-3.87%
Of the 100 significant isolates, gram-negative aerobic rods accounted for 92 % while gram-positive cocci accounted for the remaining 8%% of the total pathogens.
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Use of peptide counts as a weighting factor stems from the observation that as total pathogen titer in a sample increases, the number of different peptides that can be identified by proteomics increases in a predictable manner [18].
However, proper and highly reliable reference genes are needed for normalisation of data, as normalisation by total pathogen RNA in mixed host-pathogen samples is usually not possible.
However, the study of pathogen gene expression inside infected host tissue poses some problems, as there is no straightforward way of measuring the total pathogen RNA concentration.
This, combined with reductions in the size of the total pathogen population in mixtures, reduced the absolute frequency of occurrence of pathotypes with multiple infectivity genes in mixtures relative to that in the component pure stands.
Interestingly, at later stages of infection (24 hpi – 48 hpi), WT tubers exhibited mostly up-regulation of genes, whereas the + RB line displayed approximately equal numbers of up- and down-regulated genes, potentially reflecting contrasting rates of pathogen proliferation (as indicated by total pathogen reads counts, see results sections below) and corresponding host response patterns.
In total, respiratory pathogens were detected in 27.9% (95% confidence interval, 26.3-29.5%) of the samples submitted.
In total, these pathogens caused 99.55% cases of infectious diarrhea in China in 2011 [ 3].
In total 121 pathogens were cultivated including 103 gram positive cocci, 14 gram negative rods and four non fermentative bacilli.
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