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Total organic nitrogen was determined by the Kjeldhal method.
An increase in soluble total organic nitrogen was also observed at this time.
The total organic carbon, total organic nitrogen and moisture contents of the soil were 0.86, 0.074, and 10.64%, respectively, and the pH was 7.38.
Total nitrogen was measured using the Kjeldahl method, which is the standard way to determine the total organic nitrogen content of soil [54].
This further supports the previous suggestion of a minor contribution of terrestrial OM to the upwelling sediments derived from the low total organic nitrogen (TON) content and high C/N ratios [39].
The biomass composition was calculated from the measured Total Organic Carbon (TOC) and Total Organic Nitrogen (TON) of washed biomass pellets, using a TOC-L CPH/CPN analyzer (Shimadzu Benelux,'s-Hertogenbosch, The Netherlands).
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The total organic and nitrogen loading rates as well as the influent COD and N concentrations are shown in Table 1.
In the present investigation, experimental soils were rather poor in total nitrogen (organic nitrogen) (Fig. 19) in comparison to the organic matter content.
The water samples collected on Day 0 (initial) and 7 were analyzed for total phosphorus (TP), total particulate phosphorus, orthophosphate, total nitrogen (TN), organic nitrogen, ammonia nitrogen, nitrate-nitrite nitrogen, and chlorophyll-a.
During the crop growth cycle, data and soil samples (0 5 cm) were collected monthly, and the following were evaluated: total organic carbon, total nitrogen, potentially mineralisable nitrogen, microbial biomass carbon, C-CO2 emissions, temperature, moisture, metabolic quotient, and microbial quotient.
To chemically characterize the soil from each plot, we analyzed soil pH, total organic carbon, total nitrogen, and available phosphorus (N = 40 by plant species).
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