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The 20 µl reactions included 250 ng of total or small RNA, 1 µl of the primer/probe assay mixture, 10 µl of PCR Master Mix, 0.5 µl of RT enzyme mix (Life Technologies Applied Biosystems), and 3.5 µl of DEPC-treated water.

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Figure  1 shows that there were no significant differences in the expression levels of these two molecules associated with total RNA or small-RNA fractions.

This kit efficiently extracts RNAs larger than 10 nucleotides with specific protocols for either total RNA extraction or small RNA extraction.

major surgery to the lower gastrointestinal tract, such as partial or total colectomy, small bowel resection or partial or total gastrectomy.

Among the full cohort, we explored the distribution of total BML volume change (total BML volume = medial femur + lateral femur + medial tibia + lateral tibia) when stratified by tertiles based on baseline total BML volume (no or small BML volume, moderate BML volume, large BML volume).

Total small vessel density, perfused small vessel density, proportion of perfused small vessels and HI were analyzed with one-way analysis of variance followed by post hoc analysis with Tukey or Dunnett's T3 test.

Positions were determined with a simulated annealing technique by optimizing pathways that had large total intensities toward the center and those having small total or null intensities away from the center (Fig. 5b d).

This type of analysis can let small departments shine, because if a department's publications total is small, one or two highly cited papers can have a big impact.

No significant difference was observed in individual patients or in the total dataset (small fibre regions mean 6.59 kb large fibre regions mean 6.51 kb).

Treated stands are thought to maintain similar or smaller total forest carbon stocks than do untreated stands immediately after wildfire, because fuel treatments often remove more carbon than is saved through reductions in pyrogenic emissions, which limits the perceived short-term carbon benefit of forest fuel management activities [5, 9].

The algorithm was designed to minimize overlaps between pathways and to place the pathways that have a larger (or smaller) total intensity toward the center (or outside) (see the Pilot Application section).

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